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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Anal Biochem. 2011 Jun 7;417(1):97–102. doi: 10.1016/j.ab.2011.05.046

Fig. 1.

Fig. 1

Expression and affinity purification of PI4KA and PI4KB in 293T cells. (A) 293T human embryonic kidney cells were transfected with 3XFLAG-tagged PI4KA (middle lane), FLAG-PI4KB (right lane), or left untransfected (left lane). Tagged PI 4-kinases were affinity-purified by anti-FLAG antibody-conjugated magnetic beads followed by elution with 3XFLAG peptide. Eluted proteins were separated by SDS-PAGE and visualized by colloidal Coomassie Blue staining. (B) The purified PI 4-kinases were also characterized by immunoblotting with an anti-FLAG antibody.