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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Anal Biochem. 2011 Jun 7;417(1):97–102. doi: 10.1016/j.ab.2011.05.046

Fig. 2.

Fig. 2

Characterization of purified PI4KA and PI4KB in the ADP-Glo kinase assay. Affinity- purified PI4KA and PI4KB were incubated in a reaction mixture containing 100 μM ATP and 800 μM PtdIns (columns 4 and 7) or in a mixture containing 100 μM ATP and no PtdIns (columns 3 and 6). Columns 1 and 2 show the ADP-Glo luminescence signal from reactions lacking enzyme or from reactions with anti-FLAG immunoprecipitates from untransfected 293T cells, respectively. Column 5 shows the ADP-Glo signal from a mutant D1899A PI4KA lacking kinase activity. The data are means ± SD from quadruplicate measurements in a representative experiment.