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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Int J Biochem Cell Biol. 2011 May 4;43(8):1228–1239. doi: 10.1016/j.biocel.2011.04.016

Figure 9. The 3-trifluoromethyl group is critical for BTP2 activity.

Figure 9

(A) Structures of BTP2 and derivatives (BTP1; BTP2; Pyrazole: Pyr; 3-trifluoromethyl-5-methyl-pyrazole: 3T5M-P; 3-trifluoromethyl-pyrazole: 3T-P; 5-trifluoromethyl-3-methyl-pyrazole: 5T3MP; 5-trifluoromethyl-methyl-pyrazole: 5T-P; 3-methyl-5-methyl-pyrazole: 3M5M-P). (B) BMMCs were pretreated with vehicle, 2-APB (at 50 μM), or BTP analogs (at 1 μM) and then stimulated with ionomycin. Degranulation was determined as indicated in materials and methods. (C) RBL-2H3 cells were pretreated with vehicle or the indicated BTP analogs at 1 μM (left panel) or 3T5M-P at the indicated concentrations (right panel). Cells were then stimulated with ionomycin and degranulation determined as in figure 3. (D) BMMCs were pretreated with 3T5M-P (1 μM) or vehicle for 30 min, then stimulated with mouse IgE anti-DNP and DNP-HSA (100 ng/mL). Calcium responses were determined as in figure 1. Representative data of 3–6 experiments is shown. (E) Mean calcium increase. Data are expressed as the mean ± SEM of 3 independent experiments. Data are expressed as the mean ± SEM of 3 independent experiments. *,**p< 0.05 vs. vehicle.