Table 2.
Proteins Assigned and their Relative Abundances in aEPEC Compared to tEPEC (Fold Change)
| Spot Noa | Protein Assigned | Protein InformationResource Database Codeb | SequenceCoveragec, % | FoldChanged | pI/MWTheoretical | pI/MWExperimental |
|---|---|---|---|---|---|---|
| 07-100 | Hypothetical Protein (E. coli 101-1) | gi|194437497 | 23 | 1.18 | 5.65/21 | 5.38/68 |
| 07-107 | Outer Membrane Usher protein focD precursor | gi|1169721 | 4 | 5.13 | 6.72/96 | 6.18/81 |
| 07-116 | Hypothetical protein (E. coli E22) | gi|193061959 | 9 | 1.86 | 5.04/90 | 4.90/73 |
| 07-117 | Hypothetical Protein APEC01_O1R182 | gi|157412197 | 14 | 4.60 | 6.24/23 | 4.92/72 |
| 07-125 | similar to antirepressor of bacteriophage P22 [Enterobacteria phage VT2-Sakai] | gi|9633431 | 11 | 3.26 | 8.65/28 | 6.16/68 |
| 07-127 | paar (E. coli E22) | gi|193065132 | 12 | 2.25 | 5.0/49 | 6.08/68 |
| 07-193 | Beta-Lactamase SHV | gi|56463239 | 14 | 0.64 | 9.0/27 | 4.91/46 |
| 07-196 | Hypothetical Protein (E. coli E24377A) | gi|157158195 | 16 | 1.49 | 9.1/3.5 | 5.31/45 |
| 07-206 | Elongation factor Tu (E. coli CFT073) | gi|26110363 | 33 | 2.98 | 5.25/45 | 5.55/43 |
| 07-250 | OmpA | gi|157159269 | 22 | 0.81 | 5.65/37.5 | 5.93/28 |
| 07-255 | OmpA | gi|51235578 | 41 | 0.99 | 5.99/37.2 | 5.63/28 |
| 07-282 | Hypothetical protein ECs5262 (E. coli O157:H7) | gi|15834516 | 3 | 3.08 | 5.45/19 | 5.13/23 |
| 07-300 | OmpA | gi|195940407 | 29 | 1.14 | 5.98/29 | 5.64/22 |
| 07-340 | Transposase (E. coli APEC 01) | gi|117624195 | 20 | 15.15 | 9.61/33.6 | 4.67/18 |
| 07-367 | Chain A of OmpX | gi|6435772 | 44 | 1.17 | 5.04/16 | 5.43/14 |
| 07-486 | Protein GspC | gi|191171906 | 10 | 2.35 | 9.78/36 | 4.89/51 |
a
Refers to the proteins indicated in Fig. (1A).
b
Bacterial proteins.
c
The percentage of amino acid coverage (peptides observed/theoretical number from sequence data given in database).
d
Fold change means the ratio of % volume of protein spot of aEPEC and tEPEC; the expression is considered enhanced or diminished when the ratio is greater than 1.50- or lower than 0.66-fold. respectively [Wilkins et al., 2002.2003].