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Mild alkaline hydrolysis of chemically modified McB. N-type (left side) or X-type (right side) McB Δ0 was modified with NCS biotin at pH 4.0. MALDI-MS analysis of completed reactions is shown in panels A and B (see also Fig. 3). Reactions were next subjected to mild alkaline hydrolysis and products analyzed by MALDI-MS (panels C and D).
