Figure 5.

ELISpot assays (mean + 1 SD) for frequency of anti-DNA antibody–producing B cells and IgG-producing B cells in the spleens (a) and bone marrows (b) of treated and age-matched control mice at intervals after treatment. At least three mice were examined in each group. All the spleens from treated mice were harvested from high-dose–treated mice that did not have an increase in serum anti-DNA levels or any evidence of kidney disease. All the control mice had anti-DNA antibodies. Significant P values: spleen anti-DNA IgG: treated versus young or old control, P < 0.02; spleen total IgG: treated versus young control, P = 0.04; treated versus old control, P = 0.02; bone marrow anti-DNA IgG: treated versus control, P = 0.03.