FIGURE 8.

Down-regulation of cIAP1 modulates cell proliferation and cell cycle repartition. A, flow cytometry analysis of cell proliferation in HeLa cells transfected with control (si-Co) or cIAP1 (si-cIAP1) siRNA and cIAP1-encoding construct in the presence or not of zVAD-fmk 10 μm. Results: mean ± S.D. of at least three independent experiments. Statistically significant differences (**, p < 0.001, n = 5, Student's t test). B, cell proliferation was assessed by cell counting in THP1 clone transfected with empty or cIAP1 antisense (AS)-encoding construct as in Fig. 7C. Results are expressed as mean ± S.D. of at least three independent experiments. C and D, cell cycle analysis in HeLa cell transfected with Co or cIAP1-siRNA. The cell cycle is evaluated in by flow cytometry after BrdU and PI staining of cells. D, percentage of cell in G₀/G₁ phase of the cell cycle as analyzed by flow cytometry in HeLa cells transfected with control (Co) or cIAP1 siRNA or in THP1 cells transfected with empty vector (V) or an cIAP1 antisense encoding vector (AS). Mean ± S.D. of at least three independent experiments is shown. Statistically significant differences (*, p < 0.05, n = 3, Student's t test). E, flow cytometry analysis of EDu incorporation in HMEC cells transfected with control (si-Co) or cIAP1 (si-cIAP1) siRNA as in Fig. 7C. One representative experiment was shown. F, flow cytometry analysis of EDu incorporation in MEF cIAP1^−/− transfected with cIAP1 encoding construct as in Fig. 7F. One representative experiment is shown.