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. 2011 Jun 9;286(30):26450–26460. doi: 10.1074/jbc.M111.266643

FIGURE 8.

FIGURE 8.

H2S was required for maintenance of SMC differentiation. A, inhibition of CSE activity reduced the expressions of SMC differentiation marker genes. Postconfluent HASMCs were serum deprived for 96 h in the presence of H2S (100 μm) or D,L-propargylglycine (10 mm). B, SB203580-reversed H2S induced the expressions of SMC differentiation maker genes. Postconfluent HASMCs were incubated with H2S (100 μm) or p38 MAPK inhibitor SB203580 (40 μm) in the presence of serum (10%) for 96 h. H2S was supplemented every 24 h. Quantitative analysis was shown in the bottom panel. *, p < 0.05 versus all other groups. C, knockdown of CSE by siRNA attenuated the expressions of SMC differentiation marker genes. Postconfluent HASMCs were transfected with CSE-siRNA or negative siRNA (Neg-siRNA) at 100 nm for 72 h in the presence or absence of serum. *, p < 0.05 versus the same group in negative siRNA-transfected cells. D, CSE expression in SMCs was reduced when established in culture. SMCs were isolated from mesentery arteries (MA) of both WT and KO mice. *, p < 0.05. The data were from at least three independent experiments.