FIGURE 7.

Organotypic hippocampal slice cultures from P6-P7 WT (A, B) or D36 pups (C; gray background) were transfected after 4–5 DIV with Lck-GFP plus the indicated AKAP79 constructs. Cultures were fixed after 48 h and spines analyzed from confocal images. Cultures were treated with 200 μm Rp-cAMPS or Sp-cAMPS, 10 μm 11R-PKI, 100 μm N6BnzcAMP, for 2 h (gray bars) or 48 h (open bars) before fixation, as indicated. (n: number of neurons quantified from a minimum of three independent experiments for each condition). Significance of effects on spine densities was determined by ANOVA followed by Bonferroni's multiple comparison test with WT control in A for A and B and indicated as: *, p < 0.05; **, p < 0.01; ***, p < 0.0001. Bonferronis' multiple comparison test with D36 control in C was also performed following ANOVA of spine density for conditions in C (p < 0.0001 for AKAP rescue). Spine density of D36 control (C) is significantly increased compared with WT control (A) (p < 0.01, Student's t test).