FIGURE 8.

Organotypic hippocampal slice cultures from P6–7 WT pups were transfected after 4–5 DIV with Lck-GFP. Cultures were treated with 200 μm RpcAMPS, SpcAMPS, and 1 nm okadaic acid (OA) for 2–48 h, as indicated. (n: number of neurons quantified from 3 independent experiments). RpcAMPS reduces spine density during short term treatments but increases density over 48 h. SpcAMPS increases spine density during short term treatments but decreases density over 48 h. OA has no effect by itself over 24 and 48 h but prevents the decrease in spine density by the 48 h SpcAMPS treatment. *, p < 0.05; **, p < 0.01; ***, p < 0.0001: Bonferroni's multiple comparison with control following ANOVA (p < 0.0001).