FIGURE 2.

Eater-Fc binds to broad yet distinct classes of heat- or ethanol-inactivated bacteria. Shown is flow cytometry analysis of binding by 200 μm biotinylated Eater-Fc fusion protein (open black curve) or control biotinylated IgG1 and IgG-Fc (broken black or broken gray curves, respectively) when compared with secondary reagent only (gray filled curve) and unstained microbes (open gray curve). A, binding by Eater-Fc to heat-inactivated S. marcescens or ethanol-inactivated P. aeruginosa (Gram-negative Proteobacteria). B, binding by Eater-Fc to heat-inactivated E. faecalis and S. aureus (Gram-positive Firmicutes). C and D, no binding by Eater-Fc to heat-inactivated M. luteus (phylum Actinobacteria) (C) or heat-inactivated C. albicans yeast (D). Experiments were always run in parallel with positive and negative controls and repeated at least once with similar results.