FIGURE 3.

Eater-Fc binds to live Gram-positive Firmicutes. Shown is flow cytometry analysis of binding by 200 μm biotinylated Eater-Fc fusion protein (open black curve) or control biotinylated IgG1 and IgG-Fc (broken black or broken gray curves, respectively) when compared with secondary reagent only (gray filled curve) or unstained microbes (open gray curve). Upper two rows, Eater-Fc bound to live, as well as to formaldehyde-inactivated, E. faecalis and S. aureus (Phylum Firmicutes). Third row, Eater-Fc did not bind to M. luteus in any condition (Phylum Actinobacteria). These experiments were repeated two times with similar results.