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. 2011 May 25;286(30):26524–26532. doi: 10.1074/jbc.M110.214007

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Eater-Fc does not bind to live and formaldehyde-inactivated Gram-negative bacteria. Shown is flow cytometry analysis of binding by 200 μm biotinylated Eater-Fc fusion protein (open black curve) when compared with secondary reagent only (gray filled curve) or unstained microbes (open gray curve). Eater-Fc did not bind to live or formaldehyde-inactivated Proteobacteria (left and middle panels). Right panels, control binding to heat- or ethanol-inactivated Proteobacteria. EtOH-I., ethanol-inactivated. These experiments were repeated two times with similar results.