FIGURE 7.

Eater-Fc binds to live, AMP-exposed E. coli. A and B, dot plots of two-color flow cytometry analysis of live E. coli exposed at 25 °C to increasing concentrations of cecropin A (0–4 μm) for 10 min (A) or to 0.125 μm cecropin A for 2 h (B). The percentage of Eater-Fc binding of live or dead bacteria, respectively, is indicated. In A and B, bold rectangles highlight Eater-Fc binding to bacteria that were exposed to cecropin A at sublethal concentrations. The boxed bacteria were alive because they excluded PI; this was confirmed by bacterial CFU counts in one repeat experiment. Experiments were repeated twice with similar results.