Figure 1.

RT-PCR analysis of RNA from RXR-γ^–/– and WT mouse pituitaries. One microgram of total RNA was subjected to reverse transcription with random hexamers, and then to PCR with specific oligonucleotides for mouse RXR-γ1 (mRXR-γ1), mTSH-β, mGH, mTR-β1, mTR-β2, and mGAPDH as an RNA loading control. Product samples were removed at 30, 35, and 40 cycles and were analyzed on an agarose gel. The appropriately sized fragments were identified, and semiquantitative expression was compared between WT and RXR-γ^–/– mice.