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Intrinsic DNA cytidine deaminase activity of AID and A3G constructs. A and B, results from E. coli-based RifR mutation assays, with each X representing data from an independent culture. Median mutation frequencies are indicated by horizontal bars and numbers. Also shown are Western blot analyses of AID or A3G constructs from representative cultures with a nonspecific band (NSB) as a loading control.
