FIGURE 5.
Comparison of the intensities of the 8983 eV feature for Cu(I) complexes of the ScoHM chimera at Cu:P ratios of 2.7:1 (red trace) and 1:1 (blue trace) with those of genuine 2-coordinate (2-coord) (Cu(I)(His)2 peptide (22)) and 3-coordinate (3-coord) (Cu(I)(Met)2(His) in CusF (37)) systems. For example, the 2-coordinate bis-histidyl sites such as those present in the Cu(I) complex of the amyloid-β peptide (22, 38), and the bis-cysteinyl Cu(I)-binding sites of HAH1 (14) and CueR (39) exhibit intense 8983 eV transitions in normalized absorption edge data with intensities close to 1.