FIGURE 2.

Lethality of CO-releasing molecules is abolished by antioxidants. E. coli cells were grown under aerobic conditions and exposed to 250 μm CORM-2 (A) and 50 μm ALF062 (B) for 1 h, in medium containing the CO-releaser (black bars), and supplemented with hemoglobin (Hb, gray bars), glutathione (GSH, white bars), cysteine (Cys, black striped bars), and methionine (Met, gray striped bars). Survival percentage was calculated in relationship to cultures treated with the respective inactive compound. The results represent the average of two independent cultures performed in duplicate, and error bars represent the mean ± S.E. Sensitivity tests performed in cultures treated for 1 h with CO-RMs (+) and inactive forms (−) are also shown.