FIGURE 4.

Glutamate dehydrogenase activity is lower in cells treated with CORM-2. A, activity of GDH in cell extracts of E. coli treated for 1 h with iCORM-2 (black bars) and CORM-2 (white bars). The error bars represent the mean ± S.E. (*, p < 0.05). B, effect of 250 μm CORM-2 on E. coli viability of aerobically grown cells exposed to the CO releaser in the absence (black bars) and presence of glutamate (Glu, gray bars). The percentage of survival was calculated in relationship to cultures treated with the respective inactive compound, and error bars represent the mean ± S.E. Sensitivity tests performed in cultures treated, for 1 h, with CORM-2 (+) and iCORM-2 (−) are also presented.