FIGURE 2.

Agonist-induced phosphorylation of FPR2/ALX and FPR3. HEK293T cells expressing wild type FPR2/ALX (right panel) or FPR3 (left panel) were metabolically labeled with [³²P]orthophosphoric acid and incubated for 15 min at 37 °C with no agonist (first lane), 1 μm WKYMVm (second lane), or 1 μm F2L (third lane). After cell lysis, receptors were immunoprecipitated with an antibody directed against the last 10 amino acids of FPR2/ALX that cross-reacts with FPR3. Immunoprecipitates were treated with 2-fold Laemmli sample buffer and subjected to SDS-PAGE and autoradiography. Data are representative of three independent experiments.