FIGURE 5.

Desensitization of calcium mobilization by wild type FPR2/ALX and phosphorylation-deficient mutant FPR2-ABC. HL60 cells expressing FPR2/ALX (A) and FPR2-ABC (B) were analyzed for agonist-induced desensitization of calcium mobilization. Fura-2-loaded cells were incubated with either 100 nm WKYMVm (black symbols) or vehicle (clear symbols) for 10 min at 37 °C. Cells were then washed three times at room temperature and subsequently assayed for calcium mobilization in response to various doses of WKYMVm. Data expressed in percent of the maximum response are representative of three independent experiments.