FIGURE 7.

Cellular distribution of FPR2, FPR3, and chimeric receptors FPR3-R2 and FPR3-R2-ABC. HEK293 cells were transiently transfected with FPR2/ALX or FPR3 (tagged or not with 3HA) (A), or the chimeric receptors obtained by swapping C-terminal domains (B). Cells were fixed and permeabilized. The cellular distribution of receptors was visualized by labeling with either a polyclonal anti-FPR2/ALX antibody, which cross-react with FPR3, or a monoclonal anti-HA antibody followed by immunofluorescence staining with Alexa Fluor 488-conjugated secondary antibodies. Scale bar, 10 μm.