FIGURE 7.

Lipin 1α or Lipin 1β do not activate expression through the Krox20 MSE. A, HEK293 cells were transfected with vectors expressing rat Lipin 1α, 1β, or its mutated truncated forms, (Δα) and (Δβ), which were detected by Western blot using an anti-HA antibody. B, subcellular localization of HA-tagged rat Lipin 1 variants (red) was examined by confocal microscopy to compare the localization with the nuclear marker DAPI (blue). C and D, luciferase constructs containing the Krox20 myelination-associated enhancer (MSE), and driven by the hsp68 promoter, were co-transfected with expression plasmids encoding mouse Lipin 1α or Lipin 1β or its mutated truncated forms, Lipin 1Δα and Lipin1 Δβ (C), and/or Oct6 and Sox10 (D), in different cell types as indicated. Fold-induction was represented as the luciferase activity over the minimal hsp68 promoter. CMV enhancer/hsp68 promoter luciferase construct (pGL3 control) and the hsp68 promoter luciferase construct (pGL3 promoter) served as controls in these experiments (+, 100 ng/well; ++, 500 ng/well of Lipin 1α, Lipin 1β, Lipin 1Δα or Lipin1 Δβ; +, 200 ng/well of Oct6 and Sox10). Data are expressed as mean ± S.E.