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. 2000 Jul 1;106(1):63–72. doi: 10.1172/JCI9586

Figure 3.

Figure 3

Frequency of cytokine-producing donor-specific T cells in recipient WT, STAT4–/–, and STAT6–/– BALB/c mice following allogeneic heart transplantation. Mice were transplanted with C57BL/6 allogeneic hearts. Recipient spleens were removed at the time of rejection (9–10 days after transplant). Splenocytes (1.2 × 106 cells per well) were incubated with either recipient (open bars) or donor (filled bars) irradiated splenocytes. The frequencies of IFN-γ–producing T cells (a) and IL-5–producing T cells (b) were then determined using ELISPOT assay as described in Methods. The results are expressed as the mean number of spots per well ± SE obtained from three to four mice tested individually in each group. P values of syngeneic versus alloreactive responder frequencies in each animal group are as follows. IFN-γ: P = 0.0196, 0.0132, and 0.006 in WT, STAT4–/–, and STAT6–/– recipients, respectively. IL-5: P = 0.2327, 0.0092, and 0.0942 in WT, STAT4–/–, and STAT6–/– recipients, respectively. AP = 0.002; BP = 0.0493.