FIGURE 4.

Binding of HCRs to neuronal plasma membranes. Rat E18 primary cortical neurons were incubated with 40 nm HCRs for 1 h at 4 °C in DPBS. Cells were fixed and stained for bound HCR as described in Fig. 1D. CTB, surface bound cholera toxin B. Representative immunofluorescence images are shown; scale bar = 20 μm.