FIGURE 7.

Colocalization of HCR/D to synaptophysin. Rat E18 primary cortical neurons were incubated with 40 nm HCRs for 5 min at 37 °C in depolarizing (56 mm K⁺) buffer. Cells were fixed, permeabilized with TritonX-100, and stained for FLAG-HCRs, synaptophysin, and Rab5 as described in Fig. 2. Overlays were generated between HCR (red) and either Rab5 or syp1 (green), with areas of positive colocalization rendered yellow. Colocalization was evaluated by measuring the correlation coefficients (28) between HCR and either syp1 or Rab5 for eight independent fields; graphs represent the average with S.E. Scale bar = 20 μm. ***, p < 0.001.