TABLE 2.

L. infantum CYP51 substrate binding and catalytic parameters

Sterol	Substrate binding (spectral response)							Catalytic parameters
	Soret band, difference spectra (nm)			Binding parameters				Michaelis-Menten (substrate conversion, I+P)a			Vmax expb
	Max	Min	Isosbestic point	ΔAmax/nmol P450	High spin content	Kd	ΔAmax/Kd	Vmax	Km	Vmax/Km	I formation	P formation
				o.u.c	%	μm		μm	μm	min−1 μm−1	min−1	min−1
Obt	390	423	408	0.103 ± 0.005	94	0.27 ± 0.01	0.38	8.10 ± 0.32	11.31 ± 0.04	0.72	0.32 ± 0.01	7.3 ± 0.2
Nls	390	423	408	0.099 ± 0.005	90	0.45 ± 0.02	0.22	10.11 ± 0.41	18.52 ± 0.07	0.55	2.02 ± 0.04	6.8 ± 0.2
Mzs	391	425	411	0.074 ± 0.004	67	0.58 ± 0.03	0.13	1.92 ± 0.07	9.11 ± 0.06	0.21	1.27 ± 0.02	0.92 ± 0.05
Lns	391	428	413	0.034 ± 0.003	31	1.42 ± 0.09	0.02	0.91 ± 0.04	12.04 ± 0.08	0.07	0.59 ± 0.03	0.26 ± 0.01
Ebr	391	428	413	0.052 ± 0.004	46	0.91 ± 0.06	0.06	NTd	NT
Dhl	391	428	413	0.043 ± 0.002	39	0.82 ± 0.05	0.05	NT	NT

^a The values were calculated from Michaelis-Menten curves.

^b Experimentally reached values.

^c Optical units.

^d NT, not tested.