FIGURE 2.

Liposomes protect LC-HCT peptides from pepsin cleavage. A, 20 μg of soluble LC-HCT without (lane 1) and with (lane 2) pepsin, 40 μg of LC-HCT in proteoliposomes (lane 3), 100 μg of LC-HCT in proteoliposomes treated with pepsin (lane 4), and 8 μg of pepsin (lane 5). Arrows indicate LC-HCT peptides protected by the liposome. B, in-gel tryptic digest of bands A and B followed by mass spectrometry suggests sequences that were protected from protease degradation in the context of the proteoliposome. The two most abundant peptides (green) overlap to one region of the LC-HCT primary sequence: residues 805–821 and 826–836.