TABLE 1.

NiEDDA and O₂ accessibility of MTSL-labeled LC-HCT single cysteine mutants

Δ∏ values are calculated from ∏ proteoliposome (PL) − ∏ soluble (sol). Percent changes represent Δ∏/∏sol × 100 and are included for those residues that showed a significant change in accessibility. Most residues decreased in both O₂ and NiEDDA accessibilities when associated with liposomes. The few exceptions all had low accessibility to O₂ in both the soluble and liposome-associated forms and are therefore not thought to lie within the membrane. Residues in the 659–681 region are in bold.

Residue	NiEDDA				O2
	∏PL	∏sol	Δ∏	Change	∏PL	∏sol	Δ∏	Change
				%				%
581	2.79	4.29	−1.5	−34.9	0.84	1.14	−0.30	−26.3
605	1.04	1.78	−0.74	−41.5	0.37	0.68	−0.31	−45.6
613	0.02	0.01	0.01		0.04	0.10	−0.06
615	1.96	4.28	−2.32	−54.2	0.78	1.47	−0.69	−46.9
631	1.04	2.52	−1.48	−58.7	0.59	0.73	−0.14	−19.1
644	0.04	0.13	−0.09		0.26	0.10	0.16
647	1.82	2.70	−0.88	−32.6	0.80	0.83	−0.03	−3.6
654	0.43	0.90	−0.53	−58.9	0.30	0.51	−0.21	−41.2
657	0.26	0.36	−0.10	−27.8	0.51	0.19	0.32	168
662	1.34	3.17	−1.83	−57.7	0.53	0.83	−0.30	−36.1
665	0.09	0.31	−0.22	−71	0.18	0.24	−0.06	−25.0
666	0.14	0.63	−0.49	−77.8	0.17	0.34	−0.17	−50.0
667	0.61	3.15	−2.54	−80.6	0.43	1.20	−0.77	−64.1
669	3.05	4.02	−0.97	−24.1	1.02	1.33	−0.31	−23.3
670	0.57	1.59	−1.02	−64.2	0.46	0.49	−0.03	−6.1
671	1.32	3.14	−1.82	−58	0.70	0.96	−0.26	−27.1
674	1.90	5.21	−3.31	−63.5	1.04	1.48	−0.44	−29.7
679	3.37	4.12	−0.75	−18.2	1.01	1.21	−0.20	−16.7
725	0.02	0.01	0.01		0.06	0.04	0.02
750	1.55	2.21	−0.66	−29.8	0.67	0.95	−0.28	−29.5
770	0.43	1.09	−0.66	−60.6	0.29	0.43	−0.14	−32.6
774	0.81	1.66	−0.85	−51.2	0.47	0.81	−0.34	−42.0
775	0.15	0.37	−0.22	−59.5	0.21	0.27	−0.06	−22.2
817	1.50	1.95	−0.45	−23.1	0.73	1.16	−0.43	−37.1
819	0.89	2.93	−2.04	−69.6	0.48	1.04	−0.56	−53.8
823	1.35	2.46	−1.11	−45.1	0.78	1.37	−0.59	−43.1
825	1.16	3.18	−2.02	−63.5	0.70	1.26	−0.56	−44.4
827	2.34	4.21	−1.87	−44.4	1.04	1.24	−0.20	−16.1
829	0.78	3.13	−2.35	−75.1	0.94	1.03	−0.09	−8.7
833	1.14	2.81	−1.67	−59.4	0.82	1.23	−0.41	−33.3
835	1.26	3.50	−2.24	−64.0	0.84	1.46	−0.62	−42.5