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Pentachrome staining of the four representative cranial sutures: posterior frontal (PF), coronal (COR), sagittal (SAG), and lambdoid (LAM) at postnatal day (p) 25. A complete closure of COR suture is observed in Twist1+/− mice, while the suture remains patent in wild-type mice. The arrow indicates the fused coronal suture in Twist1+/− mice. Scale bar: 100 μm.
