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Time course of COR suture closure in Twist1+/− mice. At day p11 pentachrome staining reveals the presence of cartilage (blue staining) between the bone plates of the COR suture of Twist1+/− mice, while no cartilage is present in wild-type mice. By day p13, pathological closure of COR suture is observed in Twist1+/− mice. Dashed lines indicate the bone plates. Scale bar: 50 μm.
