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. 2011 Jul 26;2(4):e00127-11. doi: 10.1128/mBio.00127-11

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Copyright © 2011 Brazelton et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 2 — Phylogenetic tree illustrating the relationship between AMP-forming ACS of aceticlastic methanogens and the metagenomic sequence from a Lost City carbonate chimney. The Lost City partial sequence includes 276 amino acids 65% identical and 79% similar to Methanosaeta thermophila Mthe_1413. Quartet puzzling support values are shown at nodes. Note that other aceticlastic methanogens (e.g., Methanosarcina acetivorans) utilize a different pathway featuring the enzyme acetate kinase rather than AMP-forming ACS (75).