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. 2011 Jul 15;25(14):1464–1469. doi: 10.1101/gad.2060311

Figure 3.

Figure 3.

Cohesin collaborates with condensins to shape chromosomes. (A) Chromosomes were assembled in extracts containing various levels of cohesin and condensin I (for details, see Supplemental Fig. S4A), and subjected to immunofluorescence analysis with anti-topoisomerase II (topo II). Blowup images of selected regions (indicated by the rectangles) are shown in the bottom row. Bar, 5 μm. The average distance between sister chromatids, together with standard deviation, is provided below each image (n = 22, 28, 21, 20, from left to right). (B) Chromosomes were assembled in a control extract (Δmock), an extract depleted of cohesin (Δcohesin), or Wapl (ΔWapl). To visualize cohesin left on these chromosomes, immunofluorescence was performed with an antibody against its SA1 subunit. Bar, 5 μm. (C) Chromosomes were assembled as described in B, and labeled with anti-CAP-G and anti-CAP-H2 antibodies. Bar, 5 μm.