Figure 5.

Nfib is a novel oncogene in murine SCLC. (A) Quantification of the number of soft agar colonies in the uninfected or stably expressing Nfib cell line. The values represent the mean ± standard deviation of triplicate plates. (B) Growth curve of a primary tumor cell line, either uninfected or stably expressing Nfib. (C) Wild-type MEFs either uninfected or infected with Nfib-expressing viruses were plated at a low density and assayed for colony formation. Colonies were visualized using crystal violet and quantified. Values represent the mean ± standard error of the mean (SEM) of the total number of colonies in triplicate plates of both experiments using two different MEF preparations. (D) p53^−/− MEFs either uninfected or infected with Nfib-expressing viruses were plated at a low density and assayed for colony formation. Colonies were visualized using crystal violet and quantified. Values represent the mean ± SEM of the total number of colonies in triplicate plates of both experiments using two different MEF preparations. (E) p53^−/− MEFs either uninfected or infected with Nfib-expressing viruses were plated in soft agar and assayed for anchorage-independent colony formation. Colonies were visualized using crystal violet and quantified. Values represent the mean ± SEM of the number of colonies in nine camera views of triplicate plates in experiments using two different MEF preparations. (**) P-value <0.005; (***) P-value <0.0005.