Figure 6.

Effects of Nos manipulation on larval organs. (A) Brain–ring gland complexes of 5-d-old wild-type (panel I), 10-d-old Nos-RNAi^IR-X (panel II), and Nos^mac larvae (panel III). Ring glands are circled by black dashed lines. Bars, 100 μm. (B) Imaginal disc sizes from 5-d-old wild type (left) and 10-d-old Nos-RNAi^IR-X (right). Bars, 50 μm. (C) Representative cells from fat body. White lines outline individual cells. Intracellular lipids are stained by Oil-Red-O (red). Wild-type (panel I), Nos-RNAi^IR-X (panel II), and Nos^mac (panel III) fat body tissue. Note that some vesicles are devoid of lipid (black arrow). Bars, 10 mm. (D) Average number of lipid droplets per fat cell (red bars). Error bars depict standard deviation. (E) Levels of total triacylglyceride larvae (red bar) and total protein (green bar) per larva in wild-type, Nos-RNAi^IR-X, and Nos^mac larvae. Error bars depict standard deviation.