Skip to main content
. 2011 Jul 15;25(14):1510–1527. doi: 10.1101/gad.2051011

Figure 2.

Figure 2.

Ablation of FIP200 leads to autophagic defects in mammary tumor cells. (A) Sections from mammary tumors of Ctrl-MT and CKO-MT mice were analyzed by immunohistochemistry using anti-ubiquitin (top panels) or anti-p62. Note the ubiquitin- and p62-positive aggregates in tumor cells from CKO-MT mice (red arrows, right panels), but not Ctrl-MT mice (left panels). Bars, 25 μm. (B) Mammary tumor sections of Ctrl-MT and CKO-MT mice were examined by transmission electron microscopy. Note the abnormal morphology of mitochondria in CKO-MT tumor cells (red arrows, right panel). Bar, 1 μm. (C,D) Mammary tumor cells from CKO-MT or Ctrl-MT mice were analyzed for total mitochondrial mass by MitoTracker Green staining (C) or for respiring mitochondria mass by MitoTracker Deep Red staining (D). (E) Mammary tumor cells from CKO-MT or Ctrl-MT mice were incubated in the complete or starvation medium (HBSS) for 1 h with or without 100 nM bafilomycin A1 (Baf), as indicated. The cell lysates were then prepared and analyzed by immunoblotting using anti-LC3 antibody. (F) Lysates were prepared from four tumors in four different Ctrl-MT (left four lanes) and CKO-MT (right four lanes) mice and analyzed by immunoblotting using antibodies against various proteins as indicated.