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. 2009 Dec;88(12):1119–1124. doi: 10.1177/0022034509349402

Figure 4.

Figure 4.

PDK1 is not involved in superoxide generation. (A) PDK1 inhibitor (Staurosporine) did not alter superoxide generation in neutrophils from individuals with LAP upon fMLP stimulation. Isolated neutrophils from individuals with LAP and control individuals were stimulated with fMLP (1.0 µM) or PBS for 5 min. Superoxide release was measured as the superoxide dismutase-inhibitable reduction of cytochrome c and expressed as described in Materials & Methods. (n = 5 persons per group). (B) PDK1 silencing did not alter superoxide generation in neutrophil-like HL 60 cells. Values are mean ± SEM of 3 independent experiments. Asterisk indicates statistical significance compared with non-transfected HL 60 cells (*P < 0.05).