Skip to main content
. 2009 Dec;88(12):1113–1118. doi: 10.1177/0022034509350559

Figure 4.

Figure 4.

Histology of day 11 EVPOME fabricated by large cells without or with rapamycin treatment. Hematoxylin-eosin staining. EVPOME histology of small cells similar to those is not shown. (A) 2-nM rapamycin treatment for 4 wks in a monolayer culture condition + 11days at an air-liquid (A/L) interface without rapamycin treatment. (B) 20-nM rapamycin treatment for 4 wks + 11 days at A/L interface without rapamycin treatment. (C) No rapamycin treatment (control) for 4 wks + 11 days at A/L interface without rapamycin treatment. (D) No rapamycin treatment (control) for 4 wks + 11 days at A/L interface with 20-nM rapamycin treatment. (E) 2-nM rapamycin treatment for 15 wks + 11 days at A/L interface without rapamycin treatment. (F) 20-nM rapamycin treatment for 15 wks + 11 days at A/L interface without rapamycin treatment. (G) 20-nM rapamycin treatment for 15 wks + 11 days at A/L interface with 20-nM rapamycin treatment. (H) Representative histology of day 11 EVPOME produced by unsorted, passage 2 cells (13 days + 11 days at A/L interface).