Figure 3.

Geranylgerniol (GGOH), but not farnesol (FOH), partially reverses the effects of pamidronate in NHOK. (A) NHOK were treated with the indicated combinations (10 µM PAM, 1 µM FOH, 0.5 µM GGOH, 1 µM FOH/0.5 µM GGOH) for 6 days. Typical photographs were taken (100x). (B) NHOK were seeded at 2 × 10⁴ cells per well and treated with the indicated combinations of PAM, FOH, GGOH, or FOH/GGOH at day 1. The numbers were counted after 3 and 6 days. The experiment was performed in triplicate, with bars representing standard errors. (C) NHOK were plated at 2 × 10⁴ cells per well and treated with the indicated combinations of PAM, FOH, GGOH, or FOH/GGOH at day 1. After 6 days, cells were subjected to SA-β-gal activity. Cells in 3 independent fields were counted. Error bars represent standard errors.