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. 2011 Apr;90(4):501–505. doi: 10.1177/0022034510388808

Figure 1.

Figure 1.

Colonization of S. mutans BM71 on pre-existing early-colonizer biofilms in vitro. Initial biofilms of S. gordonii Challis, A. naeslundii 12104, and S. mutans BM71 (as control) were formed in 48-well polystyrene microtiter plates. S. mutans BM71pTet was added on the washed initial biofilms and incubated for another 4 hrs. The sequentially formulated biofilms were disrupted by sonication after the planktonic cells were washed away. The TetR colonies of S. mutans BM71pTet were counted on THB agar plates supplemented with tetracycline and adjusted to cfu (105)/well. Data are the mean ± standard deviation of triplicate platings from 1 of 3 reproducible experiments. **p < 0.01.