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. Author manuscript; available in PMC: 2011 Jul 26.
Published in final edited form as: Science. 2011 Mar 24;332(6026):243–247. doi: 10.1126/science.1201475

Fig. 4.

Fig. 4

Metabolic analysis of eosinophil-deficient and hypereosinophilic mice. (A) Perigonadal fat tissues (testis attached) from IL-5 transgenic (IL-5tg) and wildtype (WT) littermate controls. (B) Fasting male 8 wk-old WT or IL-5tg littermates maintained on normal chow (NC) diet were challenged with intraperitoneal glucose and blood was sampled for glucose at times indicated. Data compiled from two independent experiments with 6–7 mice in each group. (C,D) DEXA analysis of total, lean and fat tissue composition (C) or percentage adiposity (D) in ΔdblGATA and wildtype (WT) mice on normal chow (NC) or high-fat (HF) diet for 15 wk. Data compiled from two experiments with 5–8 mice in each group. (E) Intraperitoneal glucose tolerance test in male ΔdblGATA and WT mice on HF diet for 15 wk. Data compiled from 3 independent experiments with 5–8 mice in each group. (F) Fasting blood glucose in male WT and ΔdblGATA mice maintained on HF diet for 20–22 wk. Data compiled from 2 independent experiments with 5 mice in each group. (G, H) Insulin signaling, as measured by the ratio of serine phosphorylated AKT to total AKT in adipose, muscle and liver of mice aged 24 wk on HF diet (n = 4–6 mice per genotype, 4 representative mouse adipose samples shown). (I, J) Twelve-wk old wild-type C57BL/6 mice on HF diet for 6 wk were infected with N. brasiliensis (Nippo) or unchallenged (Control) and monitored for insulin tolerance (I) and glucose tolerance (J) at the indicated times. Insulin tolerance results are normalized to baseline fasting glucose, which was statistically different between cohorts (WT control 207 mg/dL ± 6; Nippo 179 mg/dL ± 7; p < 0.05). (K) Adipose tissue collected at days 40–45 post N. brasiliensis infection or from uninfected control mice and analyzed by flow cytometry for eosinophils per g adipose (K) or percent eosinophils (WT Control 2.9% ± 0.41; Nippo 10.1% ± 0.36, p<0.01). Data (I, J, K) are representative of two independent experiments with 20–30 total mice per cohort. *p<0.05, **p<0.01 as determined using Student’s t-test (B, E, F, H–K) or ANOVA with Bonferroni’s post-test correction for multiple comparisons (C–D); error bars = SEM; n.s. = not significant.