Figure 2.

Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis gel of affinity chromatography experiments and controls, stained with Novex Colloidal Blue (Invitrogen). Lanes are as follows, left to right: sizing ladder (1), ceestatin-linked coupling gel with 100 μmol/L ceestatin (10 μL) (2), ceestatin-linked coupling gel (10 μL) (3), ceestatin-linked coupling gel with 100 μmol/L ceestatin (1 μL) (4), ceestatin-linked coupling gel (1 μL) (5), mock coupling gel (10 μL) (6), and sizing ladder (7). Note that 1 μL of SDS sample buffer solution provides an insufficient amount of protein to be visualized by this staining system (see lane 5 vs lane 3).