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. 2011 Jul 26;9(7):e1001112. doi: 10.1371/journal.pbio.1001112

Figure 5. Effect of AMPK in glucose-mediated activation of hypothalamic HIF.

Figure 5

(A&B) Western blot analyses of AMPK signaling were performed for the hypothalami harvested from C57BL/6 mice that received a 5-h intra-third ventricle infusion of glucose (Glu) versus vehicle (Veh) in the presence or absence of a prior third-ventricle injection of AICAR. β-act, β-actin. Bar graphs: Western blots were quantitated and analyzed statistically. (C&D) C57BL/6 mice received mediobasal hypothalamic injection of neuron-specific lentiviruses expressing HIF2α/HIFβ (HIF2α/β) or GFP, and simultaneously received cannula implantation into the third ventricles. After 2-wk post-surgical recovery, mice received 6-h fasting and then were injected with AICAR or vehicle (Veh) via the cannula. Mice subsequently had free access to food and were measured for food intake during 1-h (C) and 3-h (D) periods. (B–D) * p<0.05, ** p<0.01, *** p<0.001; n = 6 per group. Error bars reflect mean ± SEM.