Table 1. Plasmids and strains used in this study.
Gene target | Locus | Description | Plasmid | E. coli strains | gent polarity | B. burgdorferi mutant strains |
hrpA | bb0827 | RNA helicase | pAD55 | GCE1567 | forward | hrpA2 (GCB1164), hrpA3 (GCB1165), hrpA4 GCB1166) |
bb0826 | bb0826 | hypothetical prot | pPOH57-1 | GCE2149 | forward | bb0826-11 (GCB544) |
bb0826 | bb0826 | hypothetical prot | pPOH57-2 | GCE2150 | reverse | bb0826-3 (GCB543) |