Table 2. Effect of a mutation in the B. burgdorferi hrpA (bb0827) or bb0826 gene on infection of C3H/HeN mice.
B. burgdorferi genotype | Strain | Total micea | Day 7 Bloodb | Day 7 Infection | Day 21 Ear | Day 21 Infection | Day 35c Heart | Day 35c Bladder | Day 35c Joint | Day 35c Ear | Total sitesd | Day 35 Infection |
B31 5A4 (wt) | GCB933 | 18 | 18/18 | 100.0% | 18/18 | 100.0% | 4/4 | 4/4 | 4/4 | 4/4 | 16/16 | 100.0% |
hrpA2 | GCB1164 | 3 | 0/3 | 0.0% | 0/3 | 0.0% | 0/3 | 0/3 | 0/3 | 0/3 | 0/12 | 0.0% |
hrpA3 | GCB1165 | 3 | 0/3 | 0.0% | 0/3 | 0.0% | 0/3 | 0/3 | 0/3 | 0/3 | 0/12 | 0.0% |
hrpA4 | GCB1166 | 3 | 0/3 | 0.0% | 0/3 | 0.0% | 0/3 | 0/3 | 0/3 | 0/3 | 0/12 | 0.0% |
bb0826-3 | GCB543 | 2 | 2/2 | 100.0% | 2/2 | 100.0% | 2/2 | 2/2 | 2/2 | 2/2 | 8/8 | 100.0% |
bb0826-11 | GCB544 | 2 | 2/2 | 100.0% | 2/2 | 100.0% | 2/2 | 2/2 | 2/2 | 2/2 | 8/8 | 100.0% |
There is a large number of mice in the control group because the hrpA mutants were initially assessed as part of a larger group of mutants.
Values listed correspond to number of positive cultures/number of sites tested.
Four mice infected with B. burgdorferi 5A4 were chosen as positive controls for organ harvests at day 35.
Number of positive tissue sites/number of sites tested.