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. 2011 Jul 26;6(7):e21833. doi: 10.1371/journal.pone.0021833

Figure 5. Cultivation at the air liquid interface.

Figure 5

Frames with spider silk, seeded with MEF and HaCaT cells, are lifted on a silicone scale which is filled up with polymer fibres. The surface of the nutrient media is below the altitude of the frame. Media and frame are connected by the polymer fibres, which allow unilateral nutrient supply and metabolite evacuation by diffusion.