Skip to main content
. 2011 Jul 26;6(7):e22257. doi: 10.1371/journal.pone.0022257

Figure 4. Co-localization of NcoA4 and α-tubulin at different stages of mitosis.

Figure 4

COS cells were stained for NcoA4 (green) and α-tubulin (red) and examined by confocal microscopy. Cells were also subjected to DAPI staining for visualization of chromatin (blue). Shown are representative cells at different stages of cell division: A to C, prophase COS cell showing punctate NcoA4 staining associated with microtubules. White arrows indicate mitotic organization centers. D to F, metaphase T47D cell showing localization of NcoA4 to mitotic spindles (yellow arrow). G to I, anaphase COS cell showing localization of NcoA4 to mitotic spindles (yellow arrow). NcoA4 staining was diminished at the centrosomes at this stage. J to L, telophase COS cell showing strong NcoA4 staining at the midbodies (red arrow). Yellow indicates NcoA4-α-tubulin co-localization. The pattern of NcoA4 staining shown in these representative images were present in all cells undergoing mitosis. All images shown are of single optical slices. [Bars are 2.5 µm-4.8 µm as indicated].