Figure 1. Shikonin increases glucose uptake in L6 myotubes.

(A) Glucose uptake in differentiated L6 myotubes in response to 100 nM, 1 µM or 10 µM shikonin treatment (2 h). In (B) and (C), cells were treated with 1 µM shikonin (sh) or 1 µM insulin (ins) for 2 h or 20 h respectively). Graphs show mean ± SEM of four (A), seven (B) or three (C) experiments. Asterisks represent statistical difference as analyzed by one way ANOVA between basal and treated cells (** P<0.01 *** P<0.001).