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. 2011 Jul 26;6(7):e22390. doi: 10.1371/journal.pone.0022390

Figure 3. Development and behavioural assays demonstrating rescue of the avr-14 phenotypes.

Figure 3

A). The number of eggs that developed to adulthood on different concentrations of ivermectin. There is rescue of ivermectin sensitivity by Cel-avr-14a, Cel-avr-14b and Hco-avr-14b cDNA in the resistant DA1316 mutant. Data are presented as mean ± SEM. B) The DA1316 triple mutant transformed with Hco-avr-14b cDNA carried out fewer reversals per minute than the other lines. Reversals per minute were counted to compare WT, DA1316 (glc-1; avr-14; avr-15) and DA1316 containing either Cel-avr-14b or Hco-avr-14b cDNA behind the Cel-avr-14 promoter. The double mutant, DA1370 (avr-15; glc-1), which has mutations in glc-1 and avr-15 was also used as a comparison. * Indicates that the reversals of the line containing the cDNA from the parasite were significantly reduced compared to DA1316, WT and DA1316 containing Cel-avr-14b cDNA. No difference was detected between the reversal frequencies of WT, DA1316, DA1370 or Cel-avr-14b in DA1316, using a one-way ANOVA (Minitab). Data are presented as mean ± SEM with N = 10 worms for each line.