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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Exp Hematol. 2011 May 13;39(8):837–849. doi: 10.1016/j.exphem.2011.05.005

Figure 3.

Figure 3

MHC class II and Fas co-localization on BM cells and their relationship with CD4 T cells during BM failure. Sternebrae from C.B10 mice that received TBI + B6 LN (A–D, n=3) or TBI only (E–H, n=2) treatment were cut sagittally, fixed and stained with antibodies for mouse MHC class II (red), Fas (green), CD4 (white) and DAPI (blue). Stained wholemount sternebrae tissues were analyzed by a Zeiss LSM 510 confocal microscope. Data shown are representative views of MHC class II single staining (A and E), Fas single staining (B and F), MHC class II and Fas overlap showing co-expression/co-localization (C and G), and the close encounter of MHC class II+Fas+ cells with CD4+ T cells within the BM (D and H).